Bacillus Transcriptome analysis - SRA

SRX7389894: Bacillus Transcriptome analysis
1 ILLUMINA (Illumina HiSeq 4000) run: 10.7M spots, 3.2G bases, 1.9Gb downloads

Design: After removing the rRNA, the total RNA was interrupted to short fragments and the first-strand cDNA was synthesized with random hexamer-primer. Then the second-strand cDNA was synthesized with dATPs,dGTPs,dCTPs,dUTPs, RNase H and DNA polymerase I. The obtained fragments were purified with QiaQuick PCR extraction kit, repaired the end, added poly(A), connected the sequencing adapters and degraded the second-strand cDNA with UNG enzyme. Finally, the resulting fragment was purified by MiniElute PCR Purification Kit before PCR amplification.

Submitted by: Huaiyin Institute of Technology

Study: Bacillus Transcriptome or Gene expression

PRJNA510544 • SRP237815 • All experiments • All runs

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Transcriptome Sequencing of 9 Bacillus (Bs916) and Mutants

Sample:

SAMN10791845 • SRS5838530 • All experiments • All runs

Organism: Bacillus subtilis

Library:

Name: BsH33

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq